Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis.
Identifieur interne : 000134 ( Main/Exploration ); précédent : 000133; suivant : 000135Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis.
Auteurs : Wan Hasnidah Wan Osman [Japon] ; Bunzo Mikami [Japon] ; Naoki Saka [Japon] ; Keiko Kondo [Japon] ; Meng-I Lin [Japon] ; Takashi Nagata [Japon] ; Masato Katahira [Japon]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2019.
Descripteurs français
- KwdFr :
- Biomasse (MeSH), Bois (composition chimique), Conformation des protéines (MeSH), Coriolaceae (enzymologie), Cristallographie aux rayons X (MeSH), Glutathione transferase (composition chimique), Glutathione transferase (génétique), Glutathione transferase (métabolisme), Lignine (métabolisme), Modèles moléculaires (MeSH), Mutagenèse (MeSH), Sites de fixation (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Bois, Glutathione transferase.
- enzymologie : Coriolaceae.
- génétique : Glutathione transferase.
- métabolisme : Glutathione transferase, Lignine.
- Biomasse, Conformation des protéines, Cristallographie aux rayons X, Modèles moléculaires, Mutagenèse, Sites de fixation, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Binding Sites (MeSH), Biomass (MeSH), Coriolaceae (enzymology), Crystallography, X-Ray (MeSH), Glutathione Transferase (chemistry), Glutathione Transferase (genetics), Glutathione Transferase (metabolism), Lignin (metabolism), Models, Molecular (MeSH), Mutagenesis (MeSH), Protein Conformation (MeSH), Wood (chemistry).
- MESH :
- chemical , chemistry : Glutathione Transferase.
- chemistry : Wood.
- enzymology : Coriolaceae.
- chemical , genetics : Glutathione Transferase.
- chemical , metabolism : Glutathione Transferase, Lignin.
- Amino Acid Sequence, Binding Sites, Biomass, Crystallography, X-Ray, Models, Molecular, Mutagenesis, Protein Conformation.
Abstract
Ceriporiopsis subvermispora (C. subvermispora) is a selective degrader of lignin in the woody biomass. Glutathione S-transferases (GSTs) are multifunctional enzymes that play important roles in cellular detoxification and metabolism. The crystal structures of a GST of C. subvermispora, CsGST83044, in GSH-free and -bound forms were solved at 1.95 and 2.19 Å resolution, respectively. The structure of the GSH-bound form revealed that CsGST83044 can be categorized as an atypical-type of GST. In the GSH-bound form of CsGST83044, Asn22, Asn24, and Tyr46 are located closest to the sulfur atom and form hydrogen bonds with the thiol group. The functional mutagenesis indicated that they are critical for the enzymatic activities of CsGST83044. The critical residues of an atypical-type GST belonging to the GSTFuA class were revealed for the first time. A previous study indicated that CsGST83044 and another GST, CsGST63524, differ in substrate preference; CsGST83044 prefers smaller substrates than CsGST63524 for its esterase activity. The GSH-bound pocket of CsGST83044 turns out to be small, which may explain the preference for smaller substrates. Protein engineering of GSTs of C. subvermispora in the light of the obtained insight may pave a path in the future for utilization of the woody biomass.
DOI: 10.1016/j.ijbiomac.2019.03.199
PubMed: 30928378
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis.</title><author><name sortKey="Osman, Wan Hasnidah Wan" sort="Osman, Wan Hasnidah Wan" uniqKey="Osman W" first="Wan Hasnidah Wan" last="Osman">Wan Hasnidah Wan Osman</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mikami, Bunzo" sort="Mikami, Bunzo" uniqKey="Mikami B" first="Bunzo" last="Mikami">Bunzo Mikami</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Saka, Naoki" sort="Saka, Naoki" uniqKey="Saka N" first="Naoki" last="Saka">Naoki Saka</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kondo, Keiko" sort="Kondo, Keiko" uniqKey="Kondo K" first="Keiko" last="Kondo">Keiko Kondo</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Lin, Meng I" sort="Lin, Meng I" uniqKey="Lin M" first="Meng-I" last="Lin">Meng-I Lin</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Nagata, Takashi" sort="Nagata, Takashi" uniqKey="Nagata T" first="Takashi" last="Nagata">Takashi Nagata</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: nagata.takashi.6w@kyoto-u.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Katahira, Masato" sort="Katahira, Masato" uniqKey="Katahira M" first="Masato" last="Katahira">Masato Katahira</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: katahira@iae.kyoto-u.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30928378</idno><idno type="pmid">30928378</idno><idno type="doi">10.1016/j.ijbiomac.2019.03.199</idno><idno type="wicri:Area/Main/Corpus">000140</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000140</idno><idno type="wicri:Area/Main/Curation">000140</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000140</idno><idno type="wicri:Area/Main/Exploration">000140</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis.</title><author><name sortKey="Osman, Wan Hasnidah Wan" sort="Osman, Wan Hasnidah Wan" uniqKey="Osman W" first="Wan Hasnidah Wan" last="Osman">Wan Hasnidah Wan Osman</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Mikami, Bunzo" sort="Mikami, Bunzo" uniqKey="Mikami B" first="Bunzo" last="Mikami">Bunzo Mikami</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Saka, Naoki" sort="Saka, Naoki" uniqKey="Saka N" first="Naoki" last="Saka">Naoki Saka</name><affiliation wicri:level="4"><nlm:affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Kondo, Keiko" sort="Kondo, Keiko" uniqKey="Kondo K" first="Keiko" last="Kondo">Keiko Kondo</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Lin, Meng I" sort="Lin, Meng I" uniqKey="Lin M" first="Meng-I" last="Lin">Meng-I Lin</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Nagata, Takashi" sort="Nagata, Takashi" uniqKey="Nagata T" first="Takashi" last="Nagata">Takashi Nagata</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: nagata.takashi.6w@kyoto-u.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Katahira, Masato" sort="Katahira, Masato" uniqKey="Katahira M" first="Masato" last="Katahira">Masato Katahira</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: katahira@iae.kyoto-u.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Binding Sites (MeSH)</term><term>Biomass (MeSH)</term><term>Coriolaceae (enzymology)</term><term>Crystallography, X-Ray (MeSH)</term><term>Glutathione Transferase (chemistry)</term><term>Glutathione Transferase (genetics)</term><term>Glutathione Transferase (metabolism)</term><term>Lignin (metabolism)</term><term>Models, Molecular (MeSH)</term><term>Mutagenesis (MeSH)</term><term>Protein Conformation (MeSH)</term><term>Wood (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Bois (composition chimique)</term><term>Conformation des protéines (MeSH)</term><term>Coriolaceae (enzymologie)</term><term>Cristallographie aux rayons X (MeSH)</term><term>Glutathione transferase (composition chimique)</term><term>Glutathione transferase (génétique)</term><term>Glutathione transferase (métabolisme)</term><term>Lignine (métabolisme)</term><term>Modèles moléculaires (MeSH)</term><term>Mutagenèse (MeSH)</term><term>Sites de fixation (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Wood</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Bois</term><term>Glutathione transferase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Coriolaceae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Coriolaceae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutathione transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Transferase</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glutathione transferase</term><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Binding Sites</term><term>Biomass</term><term>Crystallography, X-Ray</term><term>Models, Molecular</term><term>Mutagenesis</term><term>Protein Conformation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Conformation des protéines</term><term>Cristallographie aux rayons X</term><term>Modèles moléculaires</term><term>Mutagenèse</term><term>Sites de fixation</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ceriporiopsis subvermispora (C. subvermispora) is a selective degrader of lignin in the woody biomass. Glutathione S-transferases (GSTs) are multifunctional enzymes that play important roles in cellular detoxification and metabolism. The crystal structures of a GST of C. subvermispora, CsGST83044, in GSH-free and -bound forms were solved at 1.95 and 2.19 Å resolution, respectively. The structure of the GSH-bound form revealed that CsGST83044 can be categorized as an atypical-type of GST. In the GSH-bound form of CsGST83044, Asn22, Asn24, and Tyr46 are located closest to the sulfur atom and form hydrogen bonds with the thiol group. The functional mutagenesis indicated that they are critical for the enzymatic activities of CsGST83044. The critical residues of an atypical-type GST belonging to the GSTFuA class were revealed for the first time. A previous study indicated that CsGST83044 and another GST, CsGST63524, differ in substrate preference; CsGST83044 prefers smaller substrates than CsGST63524 for its esterase activity. The GSH-bound pocket of CsGST83044 turns out to be small, which may explain the preference for smaller substrates. Protein engineering of GSTs of C. subvermispora in the light of the obtained insight may pave a path in the future for utilization of the woody biomass.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30928378</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>132</Volume><PubDate><Year>2019</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis.</ArticleTitle><Pagination><MedlinePgn>222-229</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(19)31760-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2019.03.199</ELocationID><Abstract><AbstractText>Ceriporiopsis subvermispora (C. subvermispora) is a selective degrader of lignin in the woody biomass. Glutathione S-transferases (GSTs) are multifunctional enzymes that play important roles in cellular detoxification and metabolism. The crystal structures of a GST of C. subvermispora, CsGST83044, in GSH-free and -bound forms were solved at 1.95 and 2.19 Å resolution, respectively. The structure of the GSH-bound form revealed that CsGST83044 can be categorized as an atypical-type of GST. In the GSH-bound form of CsGST83044, Asn22, Asn24, and Tyr46 are located closest to the sulfur atom and form hydrogen bonds with the thiol group. The functional mutagenesis indicated that they are critical for the enzymatic activities of CsGST83044. The critical residues of an atypical-type GST belonging to the GSTFuA class were revealed for the first time. A previous study indicated that CsGST83044 and another GST, CsGST63524, differ in substrate preference; CsGST83044 prefers smaller substrates than CsGST63524 for its esterase activity. The GSH-bound pocket of CsGST83044 turns out to be small, which may explain the preference for smaller substrates. Protein engineering of GSTs of C. subvermispora in the light of the obtained insight may pave a path in the future for utilization of the woody biomass.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Osman</LastName><ForeName>Wan Hasnidah Wan</ForeName><Initials>WHW</Initials><AffiliationInfo><Affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mikami</LastName><ForeName>Bunzo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saka</LastName><ForeName>Naoki</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kondo</LastName><ForeName>Keiko</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Meng-I</ForeName><Initials>MI</Initials><AffiliationInfo><Affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nagata</LastName><ForeName>Takashi</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: nagata.takashi.6w@kyoto-u.ac.jp.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Katahira</LastName><ForeName>Masato</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. Electronic address: katahira@iae.kyoto-u.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055453" MajorTopicYN="N">Coriolaceae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016296" MajorTopicYN="Y">Mutagenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ceriporiopsis subvermispora</Keyword><Keyword MajorTopicYN="N">Crystal structure</Keyword><Keyword MajorTopicYN="N">Glutathione S-transferase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>4</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30928378</ArticleId><ArticleId IdType="pii">S0141-8130(19)31760-X</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2019.03.199</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Osman, Wan Hasnidah Wan" sort="Osman, Wan Hasnidah Wan" uniqKey="Osman W" first="Wan Hasnidah Wan" last="Osman">Wan Hasnidah Wan Osman</name></region><name sortKey="Katahira, Masato" sort="Katahira, Masato" uniqKey="Katahira M" first="Masato" last="Katahira">Masato Katahira</name><name sortKey="Kondo, Keiko" sort="Kondo, Keiko" uniqKey="Kondo K" first="Keiko" last="Kondo">Keiko Kondo</name><name sortKey="Lin, Meng I" sort="Lin, Meng I" uniqKey="Lin M" first="Meng-I" last="Lin">Meng-I Lin</name><name sortKey="Mikami, Bunzo" sort="Mikami, Bunzo" uniqKey="Mikami B" first="Bunzo" last="Mikami">Bunzo Mikami</name><name sortKey="Nagata, Takashi" sort="Nagata, Takashi" uniqKey="Nagata T" first="Takashi" last="Nagata">Takashi Nagata</name><name sortKey="Saka, Naoki" sort="Saka, Naoki" uniqKey="Saka N" first="Naoki" last="Saka">Naoki Saka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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